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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in lots of tumors, which results in chromosome instability, and thus might contribute to tumorigenesis. This protein was observed to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence details is not really however out there.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This information outlines more possible optimization of mobile and immunoassay parameters to be sure the absolute best outcomes are...
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This tutorial outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the very best success are...
Antibodies bound to the blocking peptide no longer bind for the epitope over the focus on protein. By evaluating the staining from your blocked antibody as opposed to the antibody by itself, you can see which staining is certain.
Goal Facts Cyclin E belongs on the hugely conserved cyclin family, whose users are characterised by a dramatic periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation patterns which add into the temporal coordination of each and every mitotic function. Cyclin E forms a complex with and capabilities as being a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates within the G1-S phase boundary and it is degraded as cells progress by way of S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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